优德88

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腺有关病毒包装服务

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优德88(中国区)官方网站
优德88(中国区)官方网站 

腺有关病毒(Adeno-associated virus,AAV)是一类无法自主复造、无包膜的二十面体微幼病毒,其直径约20-26nm,含有4.7kb左右的线状单链DNA作为基因组。AAV习染约3周可达表白顶峰

沉组腺有关病毒载体(recombinant AAV, rAAV)是在非致病的野生型AAV基础上刷新而成的基因载体,钻研当选取的rAAV通常为AAV2型基因组与分歧的衣壳蛋白结合产生的组合型沉组腺有关病毒载体,即rAAV2/x,其中x为分歧衣壳蛋白的血清型。

自互补AAV(Self-complementary AAV, scAAV)是在rAAV的基础大将其编码区域设计成双链DNA,习染细胞后互补部门互补形成双链DNA,不需期待第二链DNA合成,因而scAAV习染后3-5天即可达到表白峰值。


优德88生物AAV产品优势

纯度高:>95% 纯度                         内毒素低:<10 EU/mL 内毒素          

周期短:10个工作日 病毒包装          空壳率低:<5% 空壳率


习染成效
优德88(中国区)官方网站
优德88(中国区)官方网站

优德88生物可提供

定造rAAV:10,000+病毒载体库,涵盖过表白/滋扰、CRISPR/Cas9、Cre-loxp系统、Tet-on/off系统、特异性表白系统。

现货rAAV:1,500+现货病毒载体库,涵盖光遗传学、化学遗传学、钙/电压批示剂、神经递质探针、Cre/Flp沉组酶等工具类AAV,满足您从示踪象征、生理调控到观察验证的全面矫捷服务需要。

 
rAAV载体常用血清型列表

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※注:以上AAV基因型均为2型。优德88生物新血清型AAV发现系统AAVneOTM持续发现新型AAV,请关注优德88生物公家号。


常见启动子列表

优德88生物有60余种特异性启动子,涉及神经系统、心血管、肝脏、肌肉、肺、脂肪、胰腺、眼等钻研领域,满足基础科研尝试需要。

优德88(中国区)官方网站

部门客户文章列表

[01] Nature medicine. (IF=53.440). Zhu L J, Li T Y, Luo C X, et al. CAPON-nNOS coupling can serve as a target for developing new anxiolytics[J]. 2014, 20(9): 1050-1054. [AAV, 焦虑]

[02] Nature medicine. (IF=53.440). Cao X, Li L P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors[J]. 2013, 19(6): 773-777. [AAV, 抑郁]

[03] Nature. (IF=49.962). Yang Y, Cui Y, Sang K, et al. Ketamine blocks bursting in the lateral habenula to rapidly relieve depression[J]. 2018, 554(7692): 317-322. [AAV, 抑郁症]

[04] Science. (IF=47.728). Ren S, Wang Y, Yue F, et al. The paraventricular thalamus is a critical thalamic area for wakefulness[J]. 2018, 362(6413): 429-434. [AAV, 醒觉]

[05] Science. (IF=47.728). Mu D, Deng J, Liu K F, et al. A central neural circuit for itch sensation[J]. 2017, 357(6352): 695-699. [AAV, 痒觉]

[06] Science. (IF=47.728). Zhou T, Zhu H, Fan Z, et al. History of winning remodels thalamo-PFC circuit to reinforce social dominance[J]. 2017, 357(6347): 162-168. [AAV, 社会行为]

[07] Science. (IF=47.728). Liu D, Gu X, Zhu J, et al. Medial prefrontal activity during delay period contributes to learning of a working memory task[J]. 2014, 346(6208): 458-463. [AAV, 进建影象]

[08] Science. (IF=47.728). Li K, Zhou T, Liao L, et al. βCaMKII in lateral habenula mediates core symptoms of depression[J]. 2013, 341(6149): 1016-1020. [AAV, 抑郁]

[09] Cell. (IF=41.582). Zhu H, Wang N, Yao L, et al. Moderate UV exposure enhances learning and memory by promoting a novel glutamate biosynthetic pathway in the brain[J]. 2018, 173(7): 1716-1727. e17. [AAV, 进建影象]

[10] Cell. (IF=41.582). Bian W J, Miao W Y, He S J, et al. Coordinated spine pruning and maturation mediated by inter-spine competition for cadherin/catenin complexes[J]. 2015, 162(4): 808-822. [AAV, 神经环路]

[11] Cell. (IF=41.582). Wu H, Yu Y, Huang H, et al. Progressive pulmonary fibrosis is caused by elevated mechanical tension on alveolar stem cells[J]. 2020, 180(1): 107-121. e17. [AAV, 呼吸系统]

[12] Circulation. (IF=29.69).Gao P, You M, Li L, et al. Salt-Induced Hepatic Inflammatory Memory Contributes to Cardiovascular Damage Through Epigenetic Modulation of SIRT3[J]. 2022 Feb;145(5):375-391. [AAV, 肝脏]

[13] Cell Metabolism. (IF=27.287). Lu K, Shi TS, Shen SY,et al. Defects in a liver-bone axis contribute to hepatic osteodystrophy disease progression[J]. 2022 Mar 1;34(3):441-457.e7. [AAV, 肝骨轴]

[14] Cell Metabolism. (IF=27.287). Ji Y X, Wang Y, Li P L, et al. A kinome screen reveals that Nemo-like kinase is a key suppressor of hepatic gluconeogenesis[J]. 2021, 33(6): 1171-1186. e9. [AAV、ADV, 肝脏]

[15] Cell Metabolism. (IF=27.287). Li Y, Xu J, Lu Y, et al. DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing[J]. 2021, 33(10): 2004-2020. e9. [AAV, 肝脏]

[16] Cell Metabolism. (IF=27.287). Xiao Y Z , Yang M , Xiao Y , et al. Reducing Hypothalamic Stem Cell Senescence Protects against Aging-Associated Physiological Decline[J]. 2020, 31(3):534-548.e5. [AAV,衰老]

[17] Cell Research. (IF=25.617). Gu X, Hu X D, Wang D Q, et al. Treatment of autosomal recessive hearing loss via in vivo CRISPR/Cas9-mediated optimized homology-directed repair in mice [J]. 2022, 0:1–4. [AAV, 遗传性耳聋]

[18] Cell Research. (IF=25.617). Liu Z, Le Q, Lv Y, et al. A distinct D1-MSN subpopulation down-regulates dopamine to promote negative emotional state[J]. 2021: 1-18. [AAV,奖赏/讨厌感情]

[19] Cell Research. (IF=25.617). Xie C, Zhang Y P, Song L, et al. Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome[J]. 2016, 26(10): 1099-1111. [AAV, 心脏]

[20] Cell Research. (IF=25.617). Ma M, Xiong W, Hu F, et al. A novel pathway regulates social hierarchy via lncRNA AtLAS and postsynaptic synapsin IIb[J]. 2020, 30(2): 105-118. [AAV,社会等级]

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